Coding

Part:BBa_K3285700

Designed by: Abhinav Masih   Group: iGEM19_IISER-Pune-India   (2019-10-21)


Lead Sequestration Protein with RBS

pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.

Usage and Biology

This is an intermediate part which can be incorporated under a promoter. The pbrD protein is known to sequester lead ions in the cytoplasm of the bacterial cell.

When put under a lead responsive promoter, the part is expected to show dosage dependent sequestration properties.

Construction

"T--IISER-Pune-India--pbrD_Gel.jpeg"

The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor BBa_K1758333. This part has been synthesised with a strong Ribosome Binding Site BBa_B0034 upstream of the coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 532
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None